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Romans 8:16 note 3

163 Such a witnessing testifies to us and assures us that we are the children of God, who possess His life; it also limits us and restricts us to a living and walk that are according to this life, in keeping with our being children of God. The Spirit witnesses to our most basic and elementary relationship with God, namely, that we are His children; it does not witness that we are His sons or His heirs. Therefore, this witnessing of the Spirit begins from the time of our spiritual birth, our regeneration.

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